Unbound MEDLINE

APP/PS1 transgenic mice treated with aluminum: an update of Alzheimer's disease model.

Abstract

There is still no animal model available that can mimic all the cognitive, behavioral, biochemical, and histopathological abnormalities observed in patients with Alzheimer's disease (AD). We undertook to consider the interaction between genetic factors, including amyloid precursor protein (APP) and presenilin-1 (PS1), and environmental factors, such as Aluminum (Al) in determining susceptibility outcomes when studying the pathogenesis of AD. In this article, we provide an AD model in APP/PS1 transgenic mice triggered by Al. The animal model was established via intracerebral ventricular microinjection of aluminum chloride once a day for 5 days in APP/PS1 transgenic mice. Twenty wild type (WT) mice and 20 APP/PS1 transgenic (TG) mice were separately divided into 2 groups (control and Al group), and a stainless steel injector with stopper was used for microinjection into the left-lateral cerebral ventricle of each mouse. The Morris water maze task was used to evaluate behavioral function of learning and memory ability on the 20th day after the last injection. This AD model's brain was analyzed by: (1) amyloid beta immunohistochemical staining; (2) Tunnel staining; (3) apoptotic rates; (4) caspase-3 gene expression. Here, decrease of cognitive ability and neural cells loss were shown in APP/PS1 transgenic mice exposed to Al, which were more extensive than those in APP/PS1 TG alone and WT mice exposed to Al alone. These findings indicate that there is a close relationship between over-expression of APP and PS1 genes and Al overload. It is also suggested that APP/PS1 TG mice exposed to Al have potential value for improving AD models.

Authors

Zhang QL, Jia L, Jiao X, Guo WL, Ji JW, Yang HL, Niu Q

Institution

Ministry of Education Key Laboratory, Department of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan, China.

Source

International journal of immunopathology and pharmacology 25:1 pg 49-58

MeSH

Aluminum
Alzheimer Disease
Amyloid beta-Protein Precursor
Animals
Apoptosis
Caspase 3
Disease Models, Animal
In Situ Nick-End Labeling
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Necrosis
Presenilin-1

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22507317