Unbound MEDLINE

Friction-based stabilization of juxtacellular recordings in freely moving rats.

Abstract

Virtually nothing is known about the activity of morphologically identified neurons in freely moving mammals. Here we describe stabilization and positioning techniques that allow juxtacellular recordings from labeled single neurons in awake, freely moving animals. This method involves the use of a friction-based device that allows stabilization of the recording pipette by friction forces. Friction is generated by a clamplike mechanism that tightens a sliding pipette holder to a preimplanted pipette guide. The interacting surfaces are smoothed to optical quality (<5-nm roughness) to enable micrometer stepping precision of the device during operation. Our method allows recordings from identified neurons in freely moving animals, and thus opens new perspectives for analyzing the role of identified neurons in the control of behavior.

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  • Authors

    Herfst L, Burgalossi A, Haskic K, Tukker JJ, Schmidt M, Brecht M

    Institution

    Bernstein Center for Computational Neuroscience, Humboldt University of Berlin, Berlin, Germany.

    Source

    Journal of neurophysiology 108:2 2012 Jul pg 697-707

    MeSH

    Action Potentials
    Animals
    Brain
    Electrodes, Implanted
    Equipment Design
    Equipment Failure Analysis
    Friction
    Microelectrodes
    Movement
    Neurons
    Rats
    Rats, Wistar

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22514297