Unbound MEDLINE

Changes in peroxidase and phenols activity in apple fruit inoculated with antagonistic Pseudomonas fluorescens isolates and Botrytis mali.

Abstract

The biocontrol activity of three isolates of Pseudomonas fluorescens against gray mold of apple fruit caused by Botrytis mali and their ability to induce biochemical defense response in apple tissue were investigated. Apple fruit (Malus domestica) wounds were inoculated with 20 microL bacterial suspension (10(8) CFU mL(-1)) of Pseudomonas fluorescens followed 24 h later by 20 microL of conidial suspension of B. mali (10(5) conidia mL(-1)). The apples were then incubated at 20 degrees C for 11 days. Lesion diameters were evaluated 6 and 10 days after pathogen inoculation. In addition to controlling gray mold, these three isolates of P. fluorescens caused increase in peroxidase activities that reached maximum levels 2-6 days after pathogen inoculation. Phenolic accumulation was increased in apple fruit treated with antagonists and inoculated with B. mali and exhibited the highest level 6-8 days after treatment. The ability of P. fluorescens to increase activities of peroxidase and levels of phenol compounds maybe one of mechanism responsible its biocontrol activity.

Links

  • Aggregator Full Text
  • Authors

    Mikani A, Etebarian HR, Aminian H

    Source

    Pakistan journal of biological sciences: PJBS 14:18 2011 Sep 15 pg 854-61

    MeSH

    Botrytis
    Fruit
    Fungi
    Malus
    Peroxidase
    Pest Control, Biological
    Phenols
    Pseudomonas fluorescens

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22518925