Abstract

An endophytic bacterium, designated strain Bacillus amyloliquefaciens CGMCC 5569 was isolated from Chinese medicinal Ginkgo biloba collected from Xuzhou, China. Both the filtrate and the ethyl acetate extract of strain CGMCC 5569 showed growth inhibition activity against the sapstain fungi Lasiodiplodia rubropurpurea, L. crassispora, and L. theobromae obviously (>65%) based on the comparison of the length of zones on the petri dish. From the ethyl acetate extract of the filtrate, the antifungal compounds were obtained as a series of lipopeptides, which including series of fengycin, surfactin and bacillomycin. It showed strong growth inhibition activity in vitro against the L. rubropurpurea, L. crassispora and L. theobromae by about 70.22%, 69.53% and 78.76%, respectively. The strong anti-sapstain fungus activity indicated that the endophytic B. amyloliquefaciens CGMCC 5569 and its bioactive components might provide an alternative bio-resource for the bio-control of sapstain.

Links

Publisher Full Text

Authors

Yuan B, Wang Z, Qin S, Zhao GH, Feng YJ, Wei LH, Jiang JH

Institution

The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.

Source

Bioresource technology 114: 2012 Jun pg 536-41

MeSH

Antifungal Agents
Ascomycota
Bacillus
Cell Survival
Ginkgo biloba
Lipopeptides
Species Specificity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22520222