Abstract

The mitogen-activated protein kinase (MAPK) phosphatases (MKPs) are important negative regulators in the MAPK signaling pathways, which play crucial roles in plant growth, regulation of development and response to environment stresses. Several MAPKs have been reported to be involved in the drought stress response, however, there is no evidence for the specific function of MKPs in drought stress. Here, a putative MKP in rice (Oryza sativa), OsIBR5, was characterized. Expression of OsIBR5 was induced by PEG6000, abscisic acid (ABA) and hydrogen peroxide (H(2)O(2)). Overexpression of OsIBR5 in tobacco plants resulted in hypersensitivity to drought and H(2)O(2) treatments. Drought and ABA-induced stomatal closure was significantly reduced in OsIBR5-overexpressing tobacco plants compared with controls. Moreover, OsIBR5 was found to interact with tobacco MAPKs SIPK and WIPK, and drought-induced WIPK activity was impaired in OsIBR5-overexpressing tobacco plants. These results indicated that OsIBR5 is a MKP which was induced by abiotic stresses and decreased tolerance to drought stress in transgenic tobacco plants.

Links

Publisher Full Text

Authors

Li Y, Feng D, Zhang D, Su J, Zhang Y, Li Z, Mu P, Liu B, Wang H, Wang J

Institution

Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, PR China. liyugezi@yahoo.com.cn

Source

Plant science : an international journal of experimental plant biology 188-189: 2012 Jun pg 10-8

MeSH

Abscisic Acid
Cell Nucleus
Cytoplasm
Droughts
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Hydrogen Peroxide
Mitogen-Activated Protein Kinase Phosphatases
Mitogen-Activated Protein Kinases
Oryza sativa
Phenotype
Plant Leaves
Plant Proteins
Plant Stomata
Plant Transpiration
Plants, Genetically Modified
Polyethylene Glycols
Signal Transduction
Stress, Physiological
Tobacco
Two-Hybrid System Techniques

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22525239