Unbound MEDLINE

Effects of Ougan juice on P450 activities using a cocktail method.

Abstract

Ougan (Citrus suavissima Hort. ex Tanaka) is an important domesticated fruit which is used medicinally in China. To date, a number of methods for its identification and chemical analysis have been studied. However, the effects of Ougan juice on CYP isozymes have not been reported. Therefore, the objective of our study was to evaluate the potential effects of Ougan juice on the CYP isozymes CYP1A2, CYP2C9, CYP2C19 and CYP2D6 in rats using a cocktail approach involving the probe drugs phenacetin, tolbutamide, omeprazole and dextromethorphan. These four probe drugs were simultaneously administered to rats after single and multiple dosing of Ougan juice by gastric irrigation. The pharmacokinetics of the probes in the plasma were simultaneously determined by HPLC-MS. The main pharmacokinetic parameters of the four probe drugs were not significantly different in rats after single dose of Ougan juice. The t1/2 and AUC(0-infinity) of phenacetin and omeprazole increased significantly and their CL(z) decreased markedly after multiple dosing of Ougan juice. However, the t1/2 of tolbutamide decreased notably, while the t1/2 of dextromethorphan was not changed. The findings of this study suggest that a single administration of Ougan juice had little effect on P450 activities while multiple administration of Ougan juice tended to inhibit CYP1A2 and CYP2C19 and induce CYP2C9, but did not influence CYP2D6.

Links

  • Publisher Full Text
  • Authors

    Lin GY, Ma JS, Xu RA, Hu LF, Wang Z, Wang XQ

    Institution

    First Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.

    Source

    Die Pharmazie 67:3 2012 Mar pg 242-6

    MeSH

    Animals
    Area Under Curve
    Beverages
    Calibration
    Chromatography, High Pressure Liquid
    Citrus
    Cytochrome P-450 Enzyme System
    Food-Drug Interactions
    Half-Life
    Indicators and Reagents
    Isoenzymes
    Limit of Detection
    Male
    Mass Spectrometry
    Pharmaceutical Preparations
    Rats
    Rats, Wistar
    Reference Standards
    Regression Analysis

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22530306