Unbound MEDLINE

OIC-A006-loaded true bone ceramic heals rabbit critical-sized segmental radial defect.

Abstract

It has been reported that OIC-A006, an osteogenically inducible compound, is able to promote osteogenesis in vitro and in vivo. In this study, we used a rabbit critical-sized segmental radial defect model (CSD) (15 mm) to analyse the osteogenic activity of OIC-A006 in non-cell-seeded tissue engineering bone substitutes. The scaffold carrier was bovine sintered bone "true bone ceramic (TBC)". OIC-A006 was delivered by PLGA (D,L-lactide-co-glycolide acid) microspheres. Drug-free PLGA microspheres and rhBMP-2-loaded PLGA microspheres were used as negative and positive control groups, respectively. Three kinds of composite were fabricated by coupling TBC, type-I collagen and the corresponding microspheres. The animals were randomly divided into 4 groups: (1) Group A: defect only, (2) group B:TBC/Collagen/drug-free-microspheres, (3) group C:TBC/Collagen/ OIC-A006-microspheres, (4) group D: TBC/Collagen/rhBMP-2-microspheres. The samples were analysed by histology, X-ray,microcomputed tomography (micro-CT), and biomechanical analyses. The results showed that OIC-A006 promoted bone regeneration to a remarkable extent. It is suggested that the application of OIC-A006 might be a valuable method in bone tissue engineering for healing large segmental defects of long bones. However, the biomechanical strength was a little inferior to that of BMPs.

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  • Publisher Full Text
  • Authors

    Shao H, Shao J, Bian H, Zhao Q, Zhou Q, Qi J, Zhu Y, Wang J, Qian N, Chen B, Deng L

    Institution

    Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and Orthopaedics, Department of Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

    Source

    Die Pharmazie 67:3 2012 Mar pg 247-52

    MeSH

    Amines
    Animals
    Biomechanics
    Bone Development
    Bone Morphogenetic Proteins
    Bone and Bones
    Ceramics
    Fluorescent Dyes
    Lactic Acid
    Male
    Microscopy, Electron, Scanning
    Microspheres
    Polyglycolic Acid
    Rabbits
    Radius
    Signal Transduction
    Tissue Engineering
    Tomography, X-Ray Computed

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22530307