Abstract

It has been reported that OIC-A006, an osteogenically inducible compound, is able to promote osteogenesis in vitro and in vivo. In this study, we used a rabbit critical-sized segmental radial defect model (CSD) (15 mm) to analyse the osteogenic activity of OIC-A006 in non-cell-seeded tissue engineering bone substitutes. The scaffold carrier was bovine sintered bone "true bone ceramic (TBC)". OIC-A006 was delivered by PLGA (D,L-lactide-co-glycolide acid) microspheres. Drug-free PLGA microspheres and rhBMP-2-loaded PLGA microspheres were used as negative and positive control groups, respectively. Three kinds of composite were fabricated by coupling TBC, type-I collagen and the corresponding microspheres. The animals were randomly divided into 4 groups: (1) Group A: defect only, (2) group B:TBC/Collagen/drug-free-microspheres, (3) group C:TBC/Collagen/ OIC-A006-microspheres, (4) group D: TBC/Collagen/rhBMP-2-microspheres. The samples were analysed by histology, X-ray,microcomputed tomography (micro-CT), and biomechanical analyses. The results showed that OIC-A006 promoted bone regeneration to a remarkable extent. It is suggested that the application of OIC-A006 might be a valuable method in bone tissue engineering for healing large segmental defects of long bones. However, the biomechanical strength was a little inferior to that of BMPs.

Links

Publisher Full Text

Authors

Shao H, Shao J, Bian H, Zhao Q, Zhou Q, Qi J, Zhu Y, Wang J, Qian N, Chen B, Deng L

Institution

Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of Traumatology and Orthopaedics, Department of Orthopaedics, Shanghai Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Source

Die Pharmazie 67:3 2012 Mar pg 247-52

MeSH

Amines
Animals
Biomechanics
Bone Development
Bone Morphogenetic Proteins
Bone and Bones
Ceramics
Fluorescent Dyes
Lactic Acid
Male
Microscopy, Electron, Scanning
Microspheres
Polyglycolic Acid
Rabbits
Radius
Signal Transduction
Tissue Engineering
Tomography, X-Ray Computed

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22530307