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Production and optimization of indole acetic acid by indigenous micro flora using agro waste as substrate.

Abstract

Indole Acetic Acid (IAA) producing bacterium was isolated from the Rhizosphere soil and identified as Rhizobium sp. and Bacillus sp., Optimization of Indole acetic acid production was carried out at different cultural conditions, such as pH, temperature and substrate with Rhizobium sp., Bacillus sp. and Rhizobium sp., produced higher amount of Indole acetic acid (6.1 mg mL(-1)) than the Bacillus sp., (4.4 mg mL(-1)) at pH 7 and 37 degrees C in the Bengal gram substrate. Partial purification of Indole acetic acid was done by Thin Layer Chromatography (TLC). In conclusion Rhizobium sp., appear to be a suitable soil microorganism for high level of IAA production.

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  • Authors

    Sudha M, Gowri RS, Prabhavathi P, Astapriya P, Devi SY, Saranya A

    Institution

    Department of Microbiology and Biochemistry, Nadar Saraswathi Arts and Science College, Theni, Tamil Nadu, India.

    Source

    Pakistan journal of biological sciences: PJBS 15:1 2012 Jan 1 pg 39-43

    MeSH

    Agriculture
    Arachis hypogaea
    Bacillus
    Chromatography, Thin Layer
    Indoleacetic Acids
    Plant Roots
    Rhizobium
    Rhizosphere
    Soil Microbiology

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    22530441