Production and optimization of indole acetic acid by indigenous micro flora using agro waste as substrate.
Indole Acetic Acid (IAA) producing bacterium was isolated from the Rhizosphere soil and identified as Rhizobium sp. and Bacillus sp., Optimization of Indole acetic acid production was carried out at different cultural conditions, such as pH, temperature and substrate with Rhizobium sp., Bacillus sp. and Rhizobium sp., produced higher amount of Indole acetic acid (6.1 mg mL(-1)) than the Bacillus sp., (4.4 mg mL(-1)) at pH 7 and 37 degrees C in the Bengal gram substrate. Partial purification of Indole acetic acid was done by Thin Layer Chromatography (TLC). In conclusion Rhizobium sp., appear to be a suitable soil microorganism for high level of IAA production.
Department of Microbiology and Biochemistry, Nadar Saraswathi Arts and Science College, Theni, Tamil Nadu, India.
SourcePakistan journal of biological sciences: PJBS 15:1 2012 Jan 1 pg 39-43
Chromatography, Thin Layer
Pub Type(s)Journal Article