Abstract

A prospective clinical cohort study was established to investigate the humoral immune response in middle ear fluids (MEF) and serum against bacterial surface proteins in children suffering from recurrent acute otitis media (rAOM) and chronic otitis media with effusion (COME), using Luminex xMAP technology. The association between the humoral immune response and the presence of Moraxella catarrhalis and Streptococcus pneumoniae in the nasopharynx and middle ear was also studied. The levels of antigen-specific IgG, IgA, and IgM showed extensive interindividual variation. No significant differences in anti-M. catarrhalis and anti-S. pneumoniae serum and MEF median fluorescence intensity (MFI) values (anti-M. catarrhalis and antipneumococcal IgG levels) were observed between the rAOM or COME groups for all antigens tested. No significant differences were observed for M. catarrhalis and S. pneumoniae colonization and serum IgG levels against the Moraxella and pneumococcal antigens. Similar to the antibody response in serum, no significant differences in IgG, IgA, and IgM levels in MEF were observed for all M. catarrhalis and S. pneumoniae antigens between OM M. catarrhalis- or S. pneumoniae-positive and OM M. catarrhalis- or S. pneumonia-negative children suffering from either rAOM or COME. Finally, results indicated a strong correlation between antigen-specific serum and MEF IgG levels. We observed no significant in vivo expressed anti-M. catarrhalis or anti-S. pneumoniae humoral immune responses using a range of putative vaccine candidate proteins. Other factors, such as Eustachian tube dysfunction, viral load, and genetic and environmental factors, may play a more important role in the pathogenesis of OM and in particular in the development of rAOM or COME.

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Authors

Verhaegh SJ, Stol K, de Vogel CP, Riesbeck K, Lafontaine ER, Murphy TF, van Belkum A, Hermans PW, Hays JP

Institution

Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam, The Netherlands. s.verhaegh@erasmusmc.nl

Source

Clinical and vaccine immunology : CVI 19:6 2012 Jun pg 914-8

MeSH

Antibodies, Bacterial
Antigens, Bacterial
Antigens, Surface
Child, Preschool
Chronic Disease
Cohort Studies
Female
Humans
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Infant
Infant, Newborn
Male
Moraxella (Branhamella) catarrhalis
Moraxellaceae Infections
Nasopharynx
Otitis Media with Effusion
Pneumococcal Infections
Prospective Studies
Recurrence
Streptococcus pneumoniae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22539468