Unbound MEDLINE

Performance of two commercially available automated immunoassays for the determination of Epstein-Barr virus serological status.

Abstract

This study evaluated the performance of two automated Vidas (V) and Liaison (L) immunoassays for Epstein-Barr virus (EBV) serology. The detection of the viral capsid antigen (VCA) IgM, the VCA/early antigen (VCA/EA) IgG, and the Epstein-Barr nuclear antigen (EBNA) IgG was assessed on 526 sera collected for routine EBV testing in immunocompetent subjects. The determination of expected EBV status (186 EBV primary infections, 183 past EBV infections, and 157 EBV-seronegative individuals) was based on results of routine laboratory enzyme immunoassays (EIAs) together with clinical data. The sensitivity and specificity of each individual marker were determined in comparison to the expected EBV status. The agreement between the V and L profiles and the expected EBV status was established through the interpretation of combinations of the different EBV markers. Statistically significant differences between the two tests were found for the specificity of the VCA IgM marker (96.2% for V versus 93.2% for L), the sensitivity of the VCA/EA IgG marker (89% for V versus 94% for L), and the specificity of the EBNA IgG marker (96.5% for V versus 74.2% for L). The results determined for the two assays with respect to overall agreement with the established expected EBV status were not significantly different (89.7% for V versus 88.2% for L), with discrepancies mainly observed in sera referenced as primary infections. These findings demonstrated the similar performances of the Vidas and the Liaison assays for the establishment of an EBV serological status using the VCA, EA, and EBNA markers.

Links

  • PMC Free PDF
  • Publisher Full Text
  • Authors

    Lupo J, Germi R, Semenova T, Buisson M, Seigneurin JM, Morand P

    Institution

    Laboratoire de Virologie, Département des Agents Infectieux, Pôle Biologie, Centre Hospitalier Universitaire Grenoble, Grenoble, France.

    Source

    Clinical and vaccine immunology : CVI 19:6 2012 Jun pg 929-34

    MeSH

    Adolescent
    Adult
    Aged
    Aged, 80 and over
    Antibodies, Viral
    Automation
    Child
    Child, Preschool
    Clinical Laboratory Techniques
    Epstein-Barr Virus Infections
    Female
    Herpesvirus 4, Human
    Humans
    Immunoassay
    Immunoglobulin G
    Immunoglobulin M
    Infant
    Male
    Middle Aged
    Sensitivity and Specificity
    Virology
    Young Adult

    Pub Type(s)

    Evaluation Studies
    Journal Article

    Language

    eng

    PubMed ID

    22539474