A peptide fraction from germinated soybean protein down-regulates PTTG1 and TOP2A mRNA expression, inducing apoptosis in cervical cancer cells.
The aim of this study was to evaluate the effect of a peptide fraction, obtained from a germinated soybean protein hydrolysate, on the viability, apoptosis and cancer related gene expression in HeLa cells. Soybean was germinated for 0-6 days and proteins were isolated from the seeds. Protein isolates, without ethanol-soluble phytochemicals, were hydrolyzed with digestive enzymes and their effect on growth in HeLa cells was evaluated. The most active hydrolysate was separated by ultrafiltration into five peptide fractions. A >10 kDa fraction was the most active against cancer cells. This fraction down-regulated PTTG1 and TOP2A mRNA expression (two genes considered as therapeutic targets) and induced apoptosis in cancer cells activating the caspase cascade and causing DNA fragmentation. Germinated soy protein isolates could be a bioactive ingredient of functional food.
Departamento de Graduados en Alimentos, Escuela Nacional de Ciencias Biologicas, I.P.N. Carpio y Plan de Ayala, Col. Sto.Tomas, 11340, Mexico, D.F.
SourceJournal of experimental therapeutics & oncology 9:4 2012 pg 255-63
DNA Topoisomerases, Type II
Real-Time Polymerase Chain Reaction
Uterine Cervical Neoplasms
Pub Type(s)Journal Article
Research Support, Non-U.S. Gov't