Abstract

The aim of this study was to evaluate the effect of a peptide fraction, obtained from a germinated soybean protein hydrolysate, on the viability, apoptosis and cancer related gene expression in HeLa cells. Soybean was germinated for 0-6 days and proteins were isolated from the seeds. Protein isolates, without ethanol-soluble phytochemicals, were hydrolyzed with digestive enzymes and their effect on growth in HeLa cells was evaluated. The most active hydrolysate was separated by ultrafiltration into five peptide fractions. A >10 kDa fraction was the most active against cancer cells. This fraction down-regulated PTTG1 and TOP2A mRNA expression (two genes considered as therapeutic targets) and induced apoptosis in cancer cells activating the caspase cascade and causing DNA fragmentation. Germinated soy protein isolates could be a bioactive ingredient of functional food.

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Authors

Robles-Ramírez Mdel C, Ramón-Gallegos E, Mora-Escobedo R, Torres-Torres N

Institution

Departamento de Graduados en Alimentos, Escuela Nacional de Ciencias Biologicas, I.P.N. Carpio y Plan de Ayala, Col. Sto.Tomas, 11340, Mexico, D.F.

Source

Journal of experimental therapeutics & oncology 9:4 2012 pg 255-63

MeSH

Antigens, Neoplasm
Apoptosis
DNA Topoisomerases, Type II
DNA-Binding Proteins
Down-Regulation
Female
Germination
HeLa Cells
Humans
Neoplasm Proteins
Peptide Fragments
RNA, Messenger
Real-Time Polymerase Chain Reaction
Soybean Proteins
Soybeans
Uterine Cervical Neoplasms

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22545419