Three-dimensional cell bioreactor coupled with high performance liquid chromatography-mass spectrometry for the affinity screening of bioactive components from herb medicine.
An efficient and convenient method, three-dimensional (3-D) cell bioreactor coupled with high performance liquid chromatography-mass spectrometry was developed for affinity screening and analysis of multiple bioactive components from herbal medicines. Cancer cells were cultured on a porous scaffold to form a 3-D cell bioreactor. After interacting with live and fixed cells, the HPLC fingerprinting chromatograms of herbal medicine extract were compared to evaluate the binding properties of herbal components on cells. Model anticancer drugs (paclitaxel and resveratrol) and non-anticancer drugs (ketoprofen and penicillin G) were chosen to investigate the feasibility. When cell-drug interaction time was 30 min, the binding degrees of paclitaxel and resveratrol (each 15 μg/ml) were 82.2±7.2% and 66.1±4.1%, and for ketoprofen and penicillin G (each 15 μg/ml) were less than 3%. This method was used to screen bioactive components from Polygonum cillinerve (Nakai) Ohwi (PCO) extract, and the binding degrees of two main components in PCO extract (10 μg/ml), aristolochic acid A and aristolochic acid B, were 63.0±5.1% and 18.8±0.9%, respectively. These results demonstrated that this method was highly specific, efficient and convenient for affinity screening and analysis of bioactive components interacted with cells.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
SourceJournal of chromatography. A 1243: 2012 Jun 22 pg 33-8
Cell Culture Techniques
Cell Line, Tumor
Chromatography, High Pressure Liquid
Drug Evaluation, Preclinical
Drugs, Chinese Herbal
Pub Type(s)Journal Article
Research Support, Non-U.S. Gov't