Abstract

We report a variety of procedures for fabricating confinement-induced polymer coatings, used to eliminate non-specific protein adsorption and to control electroosmotic flow for microchip capillary electrophoresis. The coating strategy generates relatively thick polymer wall coatings (100-700 nm) and can easily be tuned by adjusting the monomer concentration. 2-hydroxyethyl methacrylate (HEMA) polymer coating, photopatterned in microfluidic channels, effectively reduced protein non-specific adsorption and rendered high efficiency (N/m=∼3 × 10⁶) for protein separation. The coating strategy provides rapid and effective means to create robust wall coatings, with the ability to photograft various surface chemistries onto the coating. [2-(methacryloyloxy) ethyl] trimethylammonium chloride grafted HEMA coated channels showed high durability and reproducibility for generating EOF (RSD=2.6%, n=64) over a period of 15 days. Sulfobetaine methacrylate grafted HEMA coated channels allowed separation of BSA digest, 15 peaks resolved in 25s, with an average N/m of 4 × 10⁵.

Links

Publisher Full Text

Authors

He M, Zeng Y, Jemere AB, Jed Harrison D

Institution

Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2 Canada.

Source

Journal of chromatography. A 1241: 2012 Jun 8 pg 112-6

MeSH

Animals
Cattle
Electroosmosis
Electrophoresis, Microchip
Methacrylates
Peptide Fragments
Polymers
Serum Albumin, Bovine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22560350