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Preparation of icariside II-phospholipid complex and its absorption across Caco-2 cell monolayers.

Abstract

In the present study, an icariside II-phospholipid complex was prepared, and its physicochemical properties including UV spectrum, IR spectrum, differential scanning calorimetry (DSC) were tested. Furthermore, the absorption of icariside II and icariside II-phospholipid complex was compared in a Caco-2 cell culture model. The results show that icariside II-phospholipid complex could significantly increase the A-B transport of icariside II (P < 0.01), with A-B Papp of (3.92 +/- 0.50) x 10(-6) cm/s compared to control (2.05 +/- 0.18) x 10(-6) cm/s (increased by 91%). Likewise, the B-A transport of icariside II was significantly enhanced (P < 0.01), with Papp of (15.3 +/- 0.72) x 10(-6) cm/s (control) to (22.4 +/- 1.4) x 10(-6) cm/s (46% increase). Efflux ratio of icariside II decreased by 23.5% after forming icariside II-phospholipid complex. The therefore, it could be concluded that phospholipid complexation can increase the intestinal absorption of icariside II.

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  • Publisher Full Text
  • Authors

    Jin X, Zhang ZH, Sun E, Tan XB, Zhu FX, Li SL, Jia XB

    Institution

    Key Laboratory of New Drug Delivery System of Chinese Materia Medical, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, China.

    Source

    Die Pharmazie 67:4 2012 Apr pg 293-8

    MeSH

    Absorption
    Biological Transport, Active
    Caco-2 Cells
    Calorimetry, Differential Scanning
    Cell Membrane
    Chromatography, High Pressure Liquid
    Data Interpretation, Statistical
    Electric Impedance
    Flavonoids
    Humans
    Phospholipids
    Spectrophotometry, Infrared
    Spectrophotometry, Ultraviolet

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22570934