Unbound MEDLINE

Using electrophoretic exclusion to manipulate small molecules and particles on a microdevice.

Abstract

Electrophoretic exclusion, a novel separations technique that differentiates species in bulk solution using the opposing forces of electrophoretic velocity and hydrodynamic flow, has been adapted to a microscale device. Proof-of-principle experiments indicate that the device was able to exclude small particles (1 μm polystyrene microspheres) and fluorescent dye molecules (rhodamine 123) from the entrance of a channel. Additionally, differentiation of the rhodamine 123 and polystyrene spheres was demonstrated. The current studies focus on the direct observation of the electrophoretic exclusion behavior on a microchip.

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  • Publisher Full Text

    • Authors

    Kenyon SM, Weiss NG, Hayes MA

    Institution

    Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287, USA.

    Source

    Electrophoresis 33:8 2012 Apr pg 1227-35

    MeSH

    Electrophoresis, Microchip
    Equipment Design
    Microfluidic Analytical Techniques
    Micromanipulation
    Microspheres
    Particle Size
    Polystyrenes
    Rhodamine 123

    Pub Type(s)

    Journal Article
    Research Support, N.I.H., Extramural

    Language

    eng

    PubMed ID

    22589099