Genome-wide gene amplification during differentiation of neural progenitor cells in vitro.
DNA sequence amplification is a phenomenon that occurs predictably at defined stages during normal development in some organisms. Developmental gene amplification was first described in amphibians during gametogenesis and has not yet been described in humans. To date gene amplification in humans is a hallmark of many tumors. We used array-CGH (comparative genomic hybridization) and FISH (fluorescence in situ hybridization) to discover gene amplifications during in vitro differentiation of human neural progenitor cells. Here we report a complex gene amplification pattern two and five days after induction of differentiation of human neural progenitor cells. We identified several amplified genes in neural progenitor cells that are known to be amplified in malignant tumors. There is also a striking overlap of amplified chromosomal regions between differentiating neural progenitor cells and malignant tumor cells derived from astrocytes. Gene amplifications in normal human cells as physiological process has not been reported yet and may bear resemblance to developmental gene amplifications in amphibians and insects.
Department of Human Genetics, Saarland University, Homburg/Saar, Germany. firstname.lastname@example.org
SourcePloS one 7:5 2012 pg e37422
Chromosomes, Artificial, Bacterial
Comparative Genomic Hybridization
In Situ Hybridization, Fluorescence
Neural Stem Cells
Pub Type(s)Journal Article
Research Support, Non-U.S. Gov't