Unbound MEDLINE

Wnt-4 potently inhibits capillary outgrowth from rat aorta in 3D culture.

Abstract

Regulation of angiogenesis involves tight cell-to-cell and cell-to-extracellular-matrix interactions. Various reports demonstrate that the Wnt signaling pathways participate in this regulation. Using a three-dimensional aortic ring culture combined with an ex vivo retroviral infection approach, we evaluated the effects of two Wnt growth factors, Wnt-1 and Wnt-4, on the formation and growth of new capillaries. Our results show that Wnt-1 had no effect, whereas Wnt-4 was a potent inhibitor of capillary outgrowth in vitro.

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  • Publisher Full Text
  • Authors

    Jover B, Girardot D, de Courtois Roy de Vacquières F, Casellas D, Molès JP

    Source

    Fundamental & clinical pharmacology 27:5 2013 Oct pg 465-70

    MeSH

    3T3 Cells
    Angiogenesis Inhibitors
    Animals
    Aorta, Abdominal
    Capillaries
    Coculture Techniques
    Endothelium, Vascular
    Extracellular Matrix
    Glycoproteins
    Imaging, Three-Dimensional
    Immunohistochemistry
    Male
    Mice
    Neovascularization, Physiologic
    Rats
    Rats, Sprague-Dawley
    Recombinant Proteins
    Tissue Culture Techniques
    Wnt1 Protein
    Wnt4 Protein

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22607657