Abstract

To gain insights into the phenotype of voltage-gated potassium (Kv)1.1 and Kv4.2 knockout mice, we used immunohistochemistry to analyze the expression of component principal or α subunits and auxiliary subunits of neuronal Kv channels in knockout mouse brains. Genetic ablation of the Kv1.1 α subunit did not result in compensatory changes in the expression levels or subcellular distribution of related ion channel subunits in hippocampal medial perforant path and mossy fiber nerve terminals, where high levels of Kv1.1 are normally expressed. Genetic ablation of the Kv4.2 α subunit did not result in altered neuronal cytoarchitecture of the hippocampus. Although Kv4.2 knockout mice did not exhibit compensatory changes in the expression levels or subcellular distribution of the related Kv4.3 α subunit, we found dramatic decreases in the cellular and subcellular expression of specific Kv channel interacting proteins (KChIPs) that reflected their degree of association and colocalization with Kv4.2 in wild-type mouse and rat brains. These studies highlight the insights that can be gained by performing detailed immunohistochemical analyses of Kv channel knockout mouse brains.

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Authors

Menegola M, Clark E, Trimmer JS

Institution

Departments of Neurobiology, Physiology and Behavior Physiology and Membrane Biology, University of California-Davis, One Shields Avenue, Davis, CA 95616-8519, U.S.A.

Source

Epilepsia 53 Suppl 1: 2012 Jun pg 142-9

MeSH

Animals
Blotting, Western
Brain Chemistry
Calcium-Binding Protein, Vitamin D-Dependent
Coloring Agents
Fluorescent Antibody Technique
Immunohistochemistry
Kv Channel-Interacting Proteins
Kv1.1 Potassium Channel
Mice
Mice, Knockout
Mossy Fibers, Hippocampal
Potassium Channels, Voltage-Gated
Presynaptic Terminals
Shal Potassium Channels
Up-Regulation

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22612819