Unbound MEDLINE

Repeated inhalation of crack-cocaine affects spermatogenesis in young and adult mice.

Abstract

To investigate the effects of repeated crack-cocaine inhalation on spermatogenesis of pubertal and mature Balb/c mice, ten young (Y(ex)) and ten adult (A(ex)) Balb/c mice were exposed to the smoke from 5 g of crack with 57.7% of pure cocaine in an inhalation chamber, 5 days/week for 2 months. The young (Y(c)) and adult (A(c)) control animals (n = 10) were kept in a specially built and controlled animal house facility. The morphologic analysis of both testes of all animals included the analysis of quantitative and qualitative histologic parameters to assess the effect of crack-cocaine on spermatogenesis and Leydig cells. Apoptosis was determined by immunolabeling with caspase-3 antibodies. Compared to the Y(c) animals, Y(ex) animals showed a significant reduction in the number of stage VII tubules per testis (p = 0.02), Sertoli cells (p < 0.001) and elongated spermatids (p = 0.001). Comparisons between the Y(ex) and A(ex) groups identified a significant reduction in the number of Sertoli cells (p < 0.001) and round spermatids (p < 0.001) in the Y(ex) group and a significant increase in apoptotic Leydig cells (p = 0.04) in the A(ex) group. The experimental results indicate that crack-cocaine smoke inhalation induced spermatogenesis disruption in chronically exposed mice, particularly in pubertal mice.

Links

  • Publisher Full Text
  • Authors

    Pires A, Pieri P, Hage M, Santos AB, Medeiros MC, Garcia RC, Yonamine M, Hallak J, Saldiva PH, Zorzetto JC, Bueno HM

    Source

    Inhalation toxicology 24:7 2012 Jun pg 439-46

    MeSH

    Administration, Inhalation
    Aging
    Animals
    Apoptosis
    Crack Cocaine
    Male
    Mice
    Mice, Inbred BALB C
    Smoke
    Spermatogenesis
    Testis

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    22642293