Abstract

Biosynthesis has led to the development of various biomimetic approaches for the fabrication of nanoscale materials. The present study reveals a unique procedure for the biosynthesis of bactericidal silver nanoparticles (AgNPs) using a novel Streptomyces sp. BDUKAS10, an isolate of mangrove sediment. Aqueous silver nitrate (AgNO(3)) solution was treated with cell free supernatant (CFS) of the isolate to synthesize bactericidal silver nanoparticles. Initial characterization was performed by visual observation for color change to intense brown color. UV-visible spectrophotometry (UV-vis) for measuring surface plasmon resonance indicated a maximum absorption peak at 441 nm. Fourier Transform Infrared Spectroscopy (FTIR) analysis provides evidence for proteins as possible reducing, and capping agents. Energy dispersive X-ray (EDAX) spectroscopy analysis showed elemental silver as major signal. Transmission Electron Microscopy (TEM) study indicated spherical silver nanoparticles in the size range of 21-48 nm. Compared to the CFS, the biosynthesized AgNPs exemplified superior bactericidal efficacy towards the tested bacterial strains. Results from this study suggested that Streptomyces sp. BDUKAS10 can be advantageous for the synthesis of AgNPs by extracellular method in the view of sustainable and ecofriendly approach.

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Publisher Full Text

Authors

Sivalingam P, Antony JJ, Siva D, Achiraman S, Anbarasu K

Institution

Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli 24, Tamil Nadu, India.

Source

Colloids and surfaces. B, Biointerfaces 98: 2012 Oct 1 pg 12-7

MeSH

Anti-Bacterial Agents
Metal Nanoparticles
Microscopy, Electron, Transmission
Silver
Spectrophotometry, Ultraviolet
Streptomyces

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22652354