Abstract

Thiamin pyrophosphokinase (TPK) converts thiamin to its active form, thiamin diphosphate. In humans, TPK expression is down-regulated in some thiamin deficiency related syndrome, and enhanced during pregnancy. Rainbow trout are also vulnerable to thiamin deficiency in wild life and are useful models for thiamin metabolism research. We identified the tpk gene transcript including seven splice variants in the rainbow trout. Almost all cell lines and tissues examined showed co-expression of several tpk splice variants including a potentially major one at both mRNA and protein levels. However, relative to other tissues, the longest variant mRNA expression was predominant in the ovary and abundant in embryos. During embryogenesis, total tpk transcripts increased abruptly in early development, and decreased to about half of the peak shortly after hatching. In rainbow trout, the tpk transcript complex is ubiquitously expressed for all tissues and cells examined, and its increase in expression could be important in the early-middle embryonic stages. Moreover, decimated tpk expression in a hepatoma cell line relative to hepatic and gonadal cell lines appears to be consistent with previously reported down-regulation of thiamin metabolism in cancer.

Links

Publisher Full Text

Authors

Yuge S, Richter CA, Wright-Osment MK, Nicks D, Saloka SK, Tillitt DE, Li W

Institution

Department of Fisheries & Wildlife, Michigan State University, East Lansing, 48824, USA.

Source

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 163:2 2012 Oct pg 193-202

MeSH

Amino Acid Sequence
Animals
Blotting, Western
Cell Line
Molecular Sequence Data
Oncorhynchus mykiss
RNA Splicing
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Thiamin Pyrophosphokinase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22659053