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- Publisher Full Text
- Authors
- Pedersen PJ
- Viart HM
- Melander F
- Andresen TL
- Madsen R
- Clausen MH
- MESH
- Cell Line, Tumor
- Humans
- Phospholipases A2
- Phospholipids
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Substrate Specificity
- Succinic Acid
- Tocopherols
Synthesis of tocopheryl succinate phospholipid conjugates and monitoring of phospholipase A₂ activity.
Abstract
Tocopheryl succinates (TOSs) are, in contrast to tocopherols, highly cytotoxic against many cancer cells. In this study the enzyme activity of secretory phospholipase A(2) towards various succinate-phospholipid conjugates has been investigated. The synthesis of six novel phospholipids is described, including two TOS phospholipids conjugates. The studies revealed that the TOS conjugates are poor substrates for the enzyme whereas the phospholipids with alkyl and phenyl succinate moieties were hydrolyzed by the enzyme to a high extent.
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Authors
Pedersen PJ, Viart HM, Melander F, Andresen TL, Madsen R, Clausen MH
Institution
Department of Chemistry, Technical University of Denmark, Kemitorvet, Building 201, DK-2800 Kgs. Lyngby, Denmark.
Source
Bioorganic & medicinal chemistry 20:13 2012 Jul 1 pg 3972-8MeSH
Cell Line, TumorHumans
Phospholipases A2
Phospholipids
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substrate Specificity
Succinic Acid
Tocopherols
Pub Type(s)
Journal ArticleResearch Support, Non-U.S. Gov't
Language
eng
PubMed ID
22672983