Coupled control of desensitization and gating by the ligand binding domain of glutamate receptors.
The kinetics of ligand gated ion channels are tuned to permit diverse roles in cellular signaling. To follow high-frequency excitatory synaptic input, postsynaptic AMPA-type glutamate receptors must recover rapidly from desensitization. Chimeras between AMPA and the related kainate receptors demonstrate that the ligand binding domains alone control the lifetime of the desensitized state. Mutation of nonconserved amino acids in the lower lobe (domain 2) of the ligand binding domain conferred slow recovery from desensitization on AMPA receptors, and fast recovery on kainate receptors. Single-channel recordings and a correlation between the rate of deactivation and the rate of recovery across panels of mutant receptors revealed that domain 2 also controls ion channel gating. Our results demonstrate that the same mechanism that ensures fast recovery also sharpens the response of AMPA channels to synaptically released glutamate.
Leibniz-Institut für Molekulare Pharmakologie, Berlin, Germany.
SourceNeuron 74:5 2012 Jun 7 pg 845-57
Cell Line, Transformed
Ion Channel Gating
Protein Processing, Post-Translational
Protein Structure, Quaternary
Pub Type(s)Journal Article
Research Support, Non-U.S. Gov't