Abstract

Volatile anaesthetic agents have been recognized for their neuroprotective properties since the 1960s. However, little is known regarding the potential retinoprotective effects of preconditioning by anaesthetic drugs. Retinal ischemia can be modeled by permanent bilateral common carotid artery occlusion (BCCAO). Here we studied the degree of ischemic injury with preconditioning by sevoflurane in the rat retina. During the BCCAO operation and preconditioning Wistar rats were anaesthetized with 1 MAC of sevoflurane. The oxygen, carbon dioxide, and anaesthetic vapor concentration in the anaesthetizing box was monitored with a gas analyzer. We examined 4 groups: non- and preconditioning groups in control and BCCAO animals. The duration of preconditioning period was 1 h and it was performed 1 day before BCCAO. The retinas were processed for histological evaluation after 2 weeks survival to determine the cell number in the ganglion cell layer and the thickness of the whole retina and that of all retinal layers. BCCAO-induced retinal ischemic injury was ameliorated by sevoflurane preconditioning. Retinal thickness and the cell number in the ganglion cell layer were more retained in preconditioned animals after BCCAO compared to non-preconditioned group. These results suggest that preconditioning using sevoflurane could provide a new perspective in retinoprotective strategies.

Links

Publisher Full Text

Authors

Szabadfi K, Danyadi B, Kiss P, Manavalan S, Gabriel R, Reglodi D, Tamas A, Trasy D, Batai I

Institution

Department of Experimental Zoology and Neurobiology, Institute of Biology, University of Pecs, 7624 Pecs, Hungary. kriszta.szabadfi@gmail.com

Source

Journal of molecular histology 43:5 2012 Oct pg 565-9

MeSH

Animals
Carotid Arteries
Eye
Ischemia
Ischemic Preconditioning
Methyl Ethers
Rats
Rats, Wistar
Retina
Retinal Degeneration
Retinal Ganglion Cells

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22684245