Abstract

Electroporation has been used successfully to introduce macromolecules such as DNA into the chick embryo for at least 15 years. Purified plasmid DNA is microinjected into embryo and then a series of low voltage electrical pulses are applied to the embryo which allows naked DNA to enter cells. Following entrance into the cytoplasm, the DNA is transported to the nucleus where it is transiently expressed. This powerful technique is useful for studies involving overexpression, misexpression, and knockdown of genes of interest at a variety of developmental timepoints.

Links

Publisher Full Text

Authors

Belecky-Adams TL, Hudson SR, Tiwari S

Institution

Department of Biology & Center for Regenerative Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN, USA. tbadams@iupui.edu

Source

Methods in molecular biology (Clifton, N.J.) 884: 2012 pg 71-89

MeSH

Animals
Chick Embryo
DNA
Electroporation
Eye
Gene Expression Regulation, Developmental
Gene Transfer Techniques
Microinjections
Transgenes

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22688699