Abstract

In our study, we explored the bidirectional communication via soluble factors between bone cells and endotoxin-stimulated splenic lymphocytes in an in vitro coculture model that mimics the inflammatory environment. Both the ability of lymphocytes to affect differentiation and immune properties of bone cells, osteoblasts (OBL) and osteoclasts (OCL), and of bone cells to modulate cytokine and activation profile of endotoxin-stimulated lymphocytes were tested. LPS-pulsed lymphocytes enhanced OCL but inhibited OBL differentiation and increased the RANKL/OPG ratio, and, at the same time, upregulated chemotactic properties of bone cells, specifically CCL2, CCL5, and CXCL10 in OCL and CCL5 and CXCL13 in OBL. In parallel, bone cells had immunosuppressive effects by downregulating the lymphocyte expression of interleukin (IL)-1, IL-6, TNF-α and co-stimulatory molecules. OCL stimulated the production of osteoclastogenic cytokine RANKL in T lymphocytes. The anti-inflammatory effect, especially of OBL, suggests a possible compensatory mechanism to limit the inflammatory reaction during infection.

Links

Publisher Full Text

Authors

Cvija H, Kovacic N, Katavic V, Ivcevic S, Aguila HL, Marusic A, Grcevic D

Institution

Department of Physiology and Immunology, University of Zagreb School of Medicine, Salata 3, 10000 Zagreb, Croatia.

Source

Inflammation 35:5 2012 Oct pg 1618-31

MeSH

Animals
Bone Marrow Cells
Cell Differentiation
Cells, Cultured
Chemokine CCL2
Chemokine CCL5
Chemokine CXCL10
Chemokine CXCL13
Female
Inflammation
Interleukin-1
Interleukin-6
Lipopolysaccharides
Lymphocyte Activation
Lymphocytes
Mice
Mice, Inbred C57BL
Osteoblasts
Osteoclasts
Osteoprotegerin
RANK Ligand
Tumor Necrosis Factor-alpha
Up-Regulation

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22699680