The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to equine herpesvirus type 1 infection.
Abstract
Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.
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Authors
Golke A, Cymerys J, Słońska A, Dzieciatkowski T, Chmielewska A, Tucholska A, Bańbura MW
Institution
Division of Virology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Ciszewskiego 8, 02-786 Warsaw, Poland.
Source
Polish journal of veterinary sciences 15:1 2012 pg 151-3MeSH
AnimalsCell Culture Techniques
Cells, Cultured
Electric Impedance
Electrophysiological Processes
Herpesvirus 1, Equid
Mice
Mice, Inbred BALB C
Neurons
Skin
Pub Type(s)
Journal ArticleResearch Support, Non-U.S. Gov't
Language
eng
PubMed ID
22708370
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