Abstract

OBJECTIVES
BCL2L12 is a recently discovered and cloned gene from members of our research team. It is a novel member of the BCL2 gene family, members of which are implicated in different hematological malignancies. In the present study, we investigated and studied the expression profile of BCL2L12 in acute myeloid leukemia (AML).
DESIGN AND METHODS
Total RNA was isolated from peripheral blood of 67 AML patients and healthy donors. The expression profile of BCL2L12 was studied using real-time PCR methodology (SYBR Green chemistry). We also evaluated the association of the BCL2L12 mRNA expression level with clinical and pathological disease parameters, as well with disease-free survival (DFS) and overall survival (OS), using the chi-square (χ(2)) test or the Fisher's exact test, where appropriate.
RESULTS
Leukemia patients expressing high level of BCL2L12 were 3 times more likely to relapse (p=0.004) or die (p=0.007) than patients with low level of BCL2L12 expression. Additionally, statistically significant relationships were revealed between BCL2L12 expression level and CD117 expression, the presence of splenomegaly and chemotherapy response.
CONCLUSIONS
Our results suggest that BCL2L12 can be considered as a new independent prognostic and chemotherapy response marker in AML.

Links

Publisher Full Text

Authors

Thomadaki H, Floros KV, Pavlovic S, Tosic N, Gourgiotis D, Colovic M, Scorilas A

Institution

Department of Biochemistry and Molecular Biology, University of Athens, Athens, Greece.

Source

Clinical biochemistry 45:16-17 2012 Nov pg 1362-7

MeSH

Adolescent
Adult
Aged
Antineoplastic Combined Chemotherapy Protocols
Case-Control Studies
Cytarabine
Daunorubicin
Etoposide
Gene Expression
Gene Expression Regulation, Neoplastic
Hepatomegaly
Humans
Kaplan-Meier Estimate
Leukemia, Myeloid, Acute
Middle Aged
Multivariate Analysis
Muscle Proteins
Neoplasm Recurrence, Local
Prognosis
Proportional Hazards Models
Proto-Oncogene Proteins c-bcl-2
Splenomegaly
Treatment Outcome
Up-Regulation
Young Adult

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22728012