Abstract

PURPOSE
The purpose of the current experimental research was to investigate whether hydroxyzine can reduce the necrotic area in ischemia-reperfusion injury in epigastric rat skin flaps and to compare its role with cimetidine and vitamin C.
METHODS
From a total of 77 ischemic rat skin flaps, 18 were treated with normal saline, 18 with vitamin C, 18 with cimetidine and 18 with hydroxyzine before reperfusion. Flap necrotic area, neutrophils and mast cells were measured on the 7th day. Analysis of variance for multiple comparisons and post hoc Dunnett's test were used for statistical analyses.
RESULTS
The sham group of animals (n=5) showed 0% flap necrosis. The saline-treated group demonstrated 75±15.3% of necrosis. The vitamin C, cimetidine and hydroxyzine groups had 56.2 ± 24.4%, 25.8 ± 19.3%, and 33.6 ± 27.8% of flap necrosis, respectively. In addition, the number of neutrophils and mast cells were decreased in the pharmacologically treated groups compared with flaps perfused with normal saline (p<0.05).
CONCLUSION
Our data suggest that administering hydroxyzine in rat epigastric skin flaps before reperfusion may attenuate necrosis, neutrophils and mast cell counts. The beneficial effect of cimetidine was the same as hydroxyzine's but the use of vitamin C was less effective.

Authors

Georgopoulos S, Mastorakos D, Kondi-Pafiti A, Katsenis K, Arkadopoulos N, Kannas D, Archontaki M, Vestarchis N, Kokkalis G

Institution

Department of Plastic and Reconstructive Surgery, Greek Anticancer Institute, St. Savvas Hospital, Athens, Greece.

Source

Journal of B.U.ON. : official journal of the Balkan Union of Oncology 17:2 pg 377-82

MeSH

Animals
Ascorbic Acid
Cimetidine
Female
Graft Survival
Histamine H1 Antagonists
Histamine H2 Antagonists
Hydroxyzine
Injections, Intraperitoneal
Mast Cells
Necrosis
Neutrophils
Rats
Rats, Sprague-Dawley
Reperfusion Injury
Skin
Surgical Flaps
Vitamins

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22740221