Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs) are emerging as novel chemopreventive agents against a variety of cancers owing to their capability in blocking the tumor development by cellular proliferation, angiogenesis and by promoting apoptosis. The present study further explored the comparative role of a traditional NSAID, indomethacin and a newly developed coxib, etoricoxib against 9,10-dimethylbenz(a)anthracene (DMBA)-induced lung carcinogenesis in rats. Morphological and histological analysis revealed the occurrence of tumors and lesions along with constricted alveolar spaces in the DMBA treated animals which were largely corrected both by indomethacin and etoricoxib. COX-1 was found to be uniformly expressed in all the groups while COX-2 levels were raised prominently in the DMBA treated animals. Proliferation, as studied by PCNA expression was found to be markedly increased in the DMBA group as compared to the others. Increased NF-κB expression in the DMBA group was found to correct with the co-administration of NSAIDs. Also, fluorescent co-staining of the isolated lung cells revealed a significantly decreased apoptosis and altered mitochondrial membrane potential. In conclusion, these parameters indicate to the chemopreventive action of the two NSAIDs studied in lung cancer and as their mechanism of action suggests, can be achievable both by COX-dependent and COX-independent pathways.

Links

Publisher Full Text

Authors

Setia S, Sanyal SN

Institution

Department of Biophysics, Panjab University, Chandigarh, India.

Source

Molecular and cellular biochemistry 369:1-2 2012 Oct pg 75-86

MeSH

9,10-Dimethyl-1,2-benzanthracene
Animals
Anti-Inflammatory Agents, Non-Steroidal
Apoptosis
Carcinogens
Cell Proliferation
Cyclooxygenase 2
Cyclooxygenase 2 Inhibitors
Gene Expression Regulation, Neoplastic
Lung Neoplasms
Male
NF-kappa B
Neoplasms, Experimental
Proliferating Cell Nuclear Antigen
Pyridines
Rats
Sulfones

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22752388