Abstract

Microarray technology is a useful tool for nucleic acid detection and has been widely used in biology and related research fields. However, the procedure is labor intensive and time consuming. Microfluidic chip-based microarrays save time with better performance, but the low spot density and probe number limit its applications. To develop high performance microarrays with high spot density within a microchannel, a method is reported here for preparing microarrays in a capillary by generating probe droplet arrays. The probes in droplets are immobilized onto the inner wall of the capillary to form a one-dimensional probe array, and then a sample solution is introduced to hybridize with the probe array. The effect of the capillary's inner diameter was evaluated to realize a high-density probe array. The processes of array generation and probe immobilization were studied to avoid possible cross contamination. The background from probe immobilization during the array generation and incubation was quantified to assure sensitivity. Multiple sample detection was also demonstrated within one capillary. The capillary based microarray assay had high spot density, easy fabrication, fast detection, high sensitivity and multiple sample capacity.

Links

Publisher Full Text

Authors

Qu X, Wang Y, Shi Z, Fu G, Zeng X, Li X, Chen H

Institution

Pen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen 361005, PR China.

Source

Biosensors & bioelectronics 38:1 pg 342-7

MeSH

Base Sequence
DNA
Equipment Design
Microfluidic Analytical Techniques
Nucleic Acid Denaturation
Oligonucleotide Array Sequence Analysis
Oligonucleotide Probes
Sensitivity and Specificity

Pub Type(s)

Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22770905