Abstract

Previously, we showed that transcutaneous (TC) DNA immunization by applying plasmid DNA onto a mouse skin area wherein the hair follicles were induced into growth stage by plucking the hair using warm waxing induced strong and functional antigen-specific antibody responses. In the present study, using plasmids that encode β-galactosidase gene or ovalbumin (OVA) gene, we showed that this mode of TC DNA immunization not only induced specific antibody responses, but also induced antigen-specific cytotoxic T lymphocyte responses. In fact, TC DNA immunization using a plasmid that encodes OVA gene prevented the growth of OVA-expressing B16-OVA tumor cells in the immunized mice. Moreover, we provided additional evidence supporting that hair follicles are essential for this mode of TC DNA immunization.

Links

Publisher Full Text

Authors

Xiao G, Li X, Kumar A, Cui Z

Institution

The University of Texas at Austin, College of Pharmacy, Austin, TX 78723, USA.

Source

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V 82:1 2012 Sep pg 212-7

MeSH

Administration, Cutaneous
Animals
Antigens
Female
Hair Removal
Immunity, Cellular
Immunity, Humoral
Immunization
Melanoma, Experimental
Mice
Mice, Inbred C57BL
Ovalbumin
Plasmids
T-Lymphocytes, Cytotoxic
Vaccines, DNA
beta-Galactosidase

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22771558