Novel printed electrode immunosensors for Schistosoma japonicum.
To improve the sensitivity and the linear range of electrochemical immunosensor to detect Schistosoma japonicum (S. japonicum) antibody.
Carbon inks and silver/silver chloride inks were printed on a polyethylene terephthalate (PET) board to make a two-electrode test strip, where carbon was the working electrode and S. japonicum soluble egg antigen (SEA) was fixed at one end of working electrode by different methods; silver/ silver chloride electrode was used as control. We tested the valency of the antibody by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) in an electrochemistry workstation, and conducted comparison with the results of ELISA. Two new immunosensing electrodes have been developed, based on glutaraldehyde cross-linked (GA) or chitosan-glutaraldehyde cross-linked (Chit-GA) transducer fixing S. japonicum antigen. We tested the titer of the antibody by means of CV and DPV.
Our experimental S. japonicum antigen (50 μg/L) is the optimal test concentration for the GA sensor, and 10 μg/L for Chit-GA sensors. The immune reaction time of both electrodes is all essentially complete in 1 minute. The linear range for S. japonicum antibody in human positive serum sample detection by the glutaraldehyde cross-linked immunosensor is 1:1000 to 1:400, and by the chitosan-glutaraldehyde cross-linked immunosensor is 1:1000 to 1:500. As the concentration of dilution ratio of S. japonicum antibody in human positive serum sample increased, the test value of DPV increased proportionally.
GA sensor and Chit-GA cross-linked S. japonicum sensors have high sensitivity and broad linear range response, and both exhibited a good linear relationship between the DPV signal and the test antibody titer.
Department of Parasitology, Medical College, Hunan Normal University, Changsha 410082, China.
SourceZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 37:6 2012 Jun pg 541-8
Pub Type(s)Journal Article
Research Support, Non-U.S. Gov't