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Effects of melatonin on testis histology, oxidative stress and spermatogenesis after experimental testis ischemia-reperfusion in rats.

Abstract

BACKGROUND
Testicular torsion due to oxidative stress results in infertility and testicular damage which can be preventable an important health problem worldwide.
AIM
The purpose of the present study was to investigate the changes of malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS) levels; histopathological alterations; morphology, concentration and motilities of the sperm in post ischemic reperfused (I/R) testis tissue.
MATERIALS AND METHODS
Forty adult male Wistar rats were carried out and were randomized to five groups; (1) Control group, (2) Ipsilateral left testis ischemia, (3) Melatonin plus ipsilateral left testis ischemia, (4) Contralateral right testis ischemia, 5. Melatonin plus contralateral right testis ischemia. After 1 h ischemia and 24 h perfusion; MDA, TAS and TOS levels were measured, histopathological alterations were determined using by Johnsen's score (JS) and sperm morphology, concentration, motility were examined.
RESULTS
MDA, TAS and TOS levels of the testis tissue did not change in all groups (p > 0.05 for all). JS was decreased in I/R group and melatonin treatment reversed histopathological changes and increased JS both in ipsilateral and contralateral testis. Abnormal sperm rate significantly increased in I/R group and melatonin administration changed abnormal sperm rate to normal.
CONCLUSIONS
As a result, the present study demonstrated that testicular damage occurs following I/R without an increase of MDA, TAS and TOS levels. Our results also suggested that melatonin is a potent antioxidant agent in preventing testicular I/R injury, as shown by increased JS and changed abnormal sperm rate.

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    • Authors

    Koksal M, Oğuz E, Baba F, Eren MA, Ciftci H, Demir ME, Kurcer Z, Take G, Aral F, Ocak AR, Aksoy N, Ulas T

    Institution

    Department of Histology and Embryology, Faculty of Medicine, Harran University, Sanliurfa, Turkey. mkoksal@harran.edu.tr

    Source

    European review for medical and pharmacological sciences 16:5 2012 May pg 582-8

    MeSH

    Animals
    Antioxidants
    Disease Models, Animal
    Male
    Malondialdehyde
    Melatonin
    Oxidative Stress
    Rats
    Rats, Wistar
    Reperfusion Injury
    Spermatic Cord Torsion
    Spermatogenesis
    Spermatozoa
    Testis

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22774397