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Regulation of inflammatory response in human chondrocytes by lentiviral mediated RNA interference against S100A10.

Abstract

OBJECTIVE
The aim of the present study was to evaluate the effects of S100A10 silencing on the inflammatory response in human chondrocytes (HCs).The inflammation induced by lipopolysaccharide (LPS) was investigated in HCs in which the S100A10 was blocked with a lentiviral shRNA vector.
METHODS
A lentiviral shRNA vector targeting S100A10 was constructed and packaged to effectively block S100A10 expression in HCs. HCs were infected with the lentivirus. S100A10 expression levels in HCs were detected by western blot analysis. Enzyme-linked immunosorbent assay (ELISA) was employed to evaluate the change of cytokine secretion levels. The effects of S100A10 silencing on the activation of mitogen-activated protein kinases (MAPKs) and NF-κB signaling pathway were also determined by western blot analysis. In addition, fluo-3-AM was used to demonstrate the change in calcium mobilization.
RESULTS
Lentivirus effectively infected the HCs and inhibited the expression of S100A10. HCs with downregulated S100A10 showed significantly decreased production of inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-10. S100A10 silencing markedly suppressed the activation of MAPKs induced by LPS. Furthermore, the calcium concentration increase in HCs stimulated by LPS was also inhibited by S100A10 knockdown.
CONCLUSION
Our investigation demonstrated that S100A10 might be considered as a potential target for anti-inflammatory treatment.

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  • Publisher Full Text

    • Authors

    Song C, Zhou X, Dong Q, Fan R, Wu G, Ji B, Meng Q, Zheng M

    Institution

    Department of Orthopaedics, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China.

    Source

    Inflammation research : official journal of the European Histamine Research Society ... [et al.] 61:11 2012 Nov pg 1219-27

    MeSH

    Adult
    Annexin A2
    Calcium
    Cell Line
    Cells, Cultured
    Chondrocytes
    Cytokines
    Genetic Vectors
    Humans
    Inflammation
    Lentivirus
    Lipopolysaccharides
    Mitogen-Activated Protein Kinases
    RNA Interference
    S100 Proteins
    Young Adult

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    22797859