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Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant.

Abstract

A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.

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    • Authors

    Schreier HJ, Dejtisakdi W, Escalante JO, Brailo M

    Institution

    Department of Marine Biotechnology, Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, Maryland, USA. Schreier@umbc.edu

    Source

    Applied and environmental microbiology 78:19 2012 Oct pg 7120-3

    MeSH

    Culture Media
    DNA Transposable Elements
    Gluconeogenesis
    Glucose
    Mutagenesis, Insertional
    Phosphoenolpyruvate Carboxykinase (ATP)
    Planctomycetales

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't
    Research Support, U.S. Gov't, Non-P.H.S.

    Language

    eng

    PubMed ID

    22798371