Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant.
A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.
Department of Marine Biotechnology, Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, Maryland, USA. Schreier@umbc.edu
SourceApplied and environmental microbiology 78:19 2012 Oct pg 7120-3
DNA Transposable Elements
Phosphoenolpyruvate Carboxykinase (ATP)
Pub Type(s)Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.