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Expression and significance of microsomal prostaglandin synthase-1 (mPGES-1) and Beclin-1 in the development of prostate cancer.

Abstract

The aim of this study was to investigate the expression and significance of microsomal prostaglandin synthase-1 (mPGES-1) and Beclin-1 in the development of prostate cancer (PCa). Immunohistochemistry was performed on paraffin-embedded sections with rabbit polyclonal against mPGES-1 and Beclin-1 in 40 PCa, 40 benign prostatic hyperplasia (BPH) and 10 normal prostate specimens for this purpose. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied for mRNA expression of mPGES-1 and Beclin-1, while MTT assays were used to ascertain the best working concentration of the mPGES-1 inhibitor (CAY10526). The effect of CAY10526 treatment on expression of Beclin-1 in DU-145 cells was studied using Western blot analysis. Localization of Beclin-1 and mPGES-1 was in endochylema. Significant differences in expression was noted among PCa, BPH and normal issues (P<0.05). Beclin-1 expression inversely correlated with mPGES-1 expression in PCa tissue (P<0.05). CAY10526 could significantly block mPGES-1 expression and the proliferation of DU-145 cells (P<0.05), while increasing Beclin-1 levels (P<0.05). Overexpression of mPGES-1 could decrease the autophagic PCa cell death. Inhibiting the expression of mPGES-1 may lead to DU-145 cell death and up-regulation of Beclin-1. The results suggest that inhibition of mPGES-1 may have therapeutic potential for PCa in the future.

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  • Publisher Full Text

    • Authors

    Xu LW, Qian M, Jia RP, Xu Z, Wu JP, Li WC, Huang WB, Chen XG

    Institution

    Department of Urology, Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, China.

    Source

    Asian Pacific journal of cancer prevention : APJCP 13:4 2012 pg 1639-44

    MeSH

    4-Butyrolactone
    Adenocarcinoma
    Age Factors
    Aged
    Aged, 80 and over
    Analysis of Variance
    Apoptosis Regulatory Proteins
    Cell Line, Tumor
    Cell Proliferation
    Cell Survival
    Chi-Square Distribution
    Humans
    Intramolecular Oxidoreductases
    Male
    Membrane Proteins
    Microsomes
    Middle Aged
    Neoplasm Grading
    Neoplasm Staging
    Prostate
    Prostatic Hyperplasia
    Prostatic Neoplasms
    Thiophenes
    Up-Regulation

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22799381