Abstract

Generally, extra-cellular-signal-regulated kinase 5 (ERK5) signaling pathway regulates many physiological activities, such as cell proliferation and cell differentiation. However, little is known about how ERKS signaling pathway composed of 15 paths participates in regulating hepatocyte proliferation during liver regeneration (LR). In this study, to explore the influence ERK5 signaling pathway upon hepatocytes at gene transcription level, rat genome 230 2.0 array was used to detect expression changes of 75 related genes in isolated hepatocytes from rat regenerating liver. Bioinformatics and systems biology methods were applied to analyze the precise role of ERK5 signaling pathway in regulating hepatocyte proliferation during LR. Results showed that 62 genes were contained in the array and 22 genes were significantly changed. It was found that 6 paths were related to hepatocyte proliferation during rat LR. Among them, paths 3, 6 and 13 of ERK5 signaling pathway modulated cell cycle progression by decreasing the negative influence on ERK5 and paths 3, 4, 8 and 9 by reinforcing the positive influence on ERK5. In summary, the study shows that 22 genes and 6 paths of ERK5 signaling pathway participate in regulating proliferation of hepatocytes in rat LR.

Authors

Li Z, Cheng Z, Wang G, Hao X, Zhang L, Xu C

Institution

College of Life Sciences, Henan Normal University, Xinxiang, Henan Province, China.

Source

Indian journal of biochemistry & biophysics 49:3 2012 Jun pg 165-72

MeSH

Animals
Cell Growth Processes
Gene Expression Profiling
Hepatectomy
Hepatocytes
Liver Regeneration
MAP Kinase Signaling System
Mitogen-Activated Protein Kinase 7
Oligonucleotide Array Sequence Analysis
Random Allocation
Rats
Rats, Sprague-Dawley

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22803331