Abstract

Fifteen essential oils of pharmaceutical grade were fingerprinted by five techniques: TLC, GC, HPLC, attenuated total reflectance FTIR spectroscopy, and differential scanning calorimetry (DSC). Denoising and baseline removal was found to be a crucial step for correct comparative analysis. Standardization of the signal was not necessary in the presented case; however, it should be considered and checked in each case. Due to small variance explained by first two principal components (below 50%) and outlying observations, the main analysis was performed by Euclidean dendrograms. It was found that almost all techniques besides DSC find real chemical similarities; however, DSC can be used as an additional tool. The similarities among the five techniques were also compared and discussed.

Links

Aggregator Full Text

Authors

Pietraś R, Skibiński R, Trebacz H, Gumieniczek A

Institution

Medical University of Lublin, Department of Medicinal Chemistry, Lublin, Poland.

Source

Journal of AOAC International 95:3 pg 699-703

MeSH

Calorimetry, Differential Scanning
Chromatography, Gas
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Oils, Volatile
Spectroscopy, Fourier Transform Infrared

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

22816259