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Application of curve resolution algorithms in the study of drug photodegradation kinetics -- the example of moclobemide.

Abstract

The photodegradation of moclobemide was studied in methanolic media. Ultra-HPLC (UHPLC)/MS/MS analysis proved decomposition to 4-chlorobenzamide as a major degradation product and small amounts of Ro 16-3177 (4-chloro-N-[2-[(2-hydroxyethyl)amino] ethyl]benzamide) and 2-[(4-chlorobenzylidene)amino]-N-[2-ethoxyethenyl]ethenamine. The methanolic solution was investigated spectrophotometrically in the UV region, registering the spectra during 30 min of degradation. Using reference spectra and a multivariate chemometric method (multivariate curve resolution-alternating least squares), the spectra were resolved and concentration profiles were obtained. The obtained results were in good agreement with a quantitative approach, with UHPLC-diode array detection as the reference method.

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  • Aggregator Full Text

    • Authors

    Skibiński R, Komsta Ł

    Institution

    robert.skibinski@am.lublin.pl

    Source

    Journal of AOAC International 95:3 pg 708-12

    MeSH

    Algorithms
    Benzamides
    Chromatography, High Pressure Liquid
    Kinetics
    Moclobemide
    Photolysis
    Tandem Mass Spectrometry

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    22816261