Unbound MEDLINE

Comparative analysis of periprostatic implantation and intracavernosal injection of human adipose tissue-derived stem cells for erectile function recovery in a rat model of cavernous nerve injury.

Abstract

BACKGROUND
We compared periprostatic implantation (PPI) and intracavernosal injection (ICI) of human adipose tissue-derived stem cell (ADSC) to facilitate recovery of erectile function in a rat model of cavernous nerve (CN) injury.
METHODS
Bilateral CN dissection (BCND) was induced in Sprague-Dawley rats. After BCND 10 rats each were treated with PPI and/or ICI of ADSCs. After 4 weeks erectile responses to electric pelvic ganglion stimulation were studied. Each penis was evaluated in terms of the expression of neuronal nitric oxide synthase and smooth muscle content.
RESULTS
The ratio of maximal intracavernosal pressure to mean arterial pressure was significantly decreased in the BCND group (24.5%) compared to the sham group (64.2%). PPI and ICI significantly improved erectile function (46.7% and 47.9%, respectively) compared to the BCND group. A combination of PPI and ICI (42.5%) did not afford any incremental effect on erectile function. After stem cell therapy, the expression of neuronal nitric oxide synthase increased slightly in the ICI group without statistical relevance, whereas the PPI and combination groups showed marginally significant increases (P = 0.08). In both the PPI and ICI groups, the smooth muscle content was similar to the sham group. The combination group showed remarkable increase in smooth muscle content to an extent greater than that seen when either treatment was given alone, although statistically not significant.
CONCLUSION
PPI or ICI of ADSCs in a rat model of CN injury were equally effective in recovering penile erection, but may address different types of pathophysiology.

Links

  • Publisher Full Text

    • Authors

    You D, Jang MJ, Lee J, Suh N, Jeong IG, Sohn DW, Kim SW, Ahn TY, Kim CS

    Institution

    Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

    Source

    The Prostate 73:3 2013 Feb 15 pg 278-86

    MeSH

    Adipose Tissue
    Animals
    Cells, Cultured
    Electric Stimulation
    Erectile Dysfunction
    Humans
    Male
    Models, Animal
    Muscle, Smooth
    Nerve Regeneration
    Nitric Oxide Synthase Type I
    Penis
    Peripheral Nerve Injuries
    Prostate
    Prostheses and Implants
    Rats
    Rats, Sprague-Dawley
    Recovery of Function
    Stem Cell Transplantation
    Stem Cells

    Pub Type(s)

    Comparative Study
    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22821215