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Nanotechnology and mesenchymal stem cells with chondrocytes in prevention of partial growth plate arrest in pigs.

Abstract

INTRODUCTION
This study describes the results achieved using a combination of allogeneic mesenchymal stem cells (MSCs) with chondrocytes (CHC) and a new scaffold consisting of type-I collagen and chitosan nanofibers in the prevention of partial growth plate arrest after iatrogenic injury in pigs.
MATERIAL AND METHODS
The miniature pig was selected as an experimental model to compare the results in the left femoral bones (MSCs and CHC in scaffold transplantation into the iatrogenic partial distal growth plate defect) and right femoral bones (scaffold alone transplantation). The experimental group consisted of 10 animals. Bone marrow from os ilium as the source of MSCs was used. A porous cylinder consisting of 0.5% by weight type-I collagen and 30% by weight chitosan, was the optimal choice. The length of the bone and angular deformity of distal femur after the healing period was measured and the quality and structure of the newly formed cartilage was histologically examined.
RESULTS
Transplantation of the composite scaffold in combination with MSCs and chondrocytes led to the prevention of growth disorder and angular deformity in the distal epiphysis of the left femur. Compared to the right (control) femur, tissue similar to hyaline cartilage with signs of columnar organization typical of the growth plate occurred in most cases.
CONCLUSIONS
The promising results of this study reveal the new and effective means for the prevention of bone bridge formation after growth plate injury.

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  • Publisher Full Text

    • Authors

    Planka L, Srnec R, Rauser P, Stary D, Filova E, Jancar J, Juhasova J, Kren L, Necas A, Gal P

    Institution

    Clinic of Pediatric Surgery, Orthopedics and Traumatology, Faculty Hospital Brno, Czech Republic. lplanka@seznam.cz

    Source

    Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia 156:2 2012 Jun pg 128-34

    MeSH

    Animals
    Cartilage, Articular
    Chitosan
    Chondrocytes
    Epiphyses
    Femur
    Growth Plate
    Mesenchymal Stem Cell Transplantation
    Nanofibers
    Swine
    Swine, Miniature
    Tissue Engineering
    Tissue Scaffolds

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    22837133