Abstract

BACKGROUND
Lack of safe and efficient delivery of siRNA remains the greatest hurdle for the therapeutic application of siRNA. This article reports synthesis and evaluation of novel lipoidal amine-based nanocarrier (LANC) formulations for siRNA delivery.
METHOD
Physicochemical properties were analyzed for LANC formulations. siRNA delivery efficiency of LANC-siRNA complexes was determined using a luciferase reporter gene assay. Cytotoxicity of the LANC-siRNA complexes was measured by the MTS assay. Finally, cellular uptake and cytoplasmic release of siRNA were analyzed using flow cytometry.
CONCLUSION
The LANC formulation facilitated siRNA uptake and release into the cytoplasm, mediating significant luciferase knockdown (70% inhibition).

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Authors

Zhou C, Yue Z, Lee LJ, Lee RJ

Institution

Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.

Source

Therapeutic delivery 3:6 2012 Jun pg 715-23

MeSH

Biological Transport
Cell Line, Tumor
Cell Survival
Cytoplasm
Diamines
Flow Cytometry
Gene Therapy
Genes, Reporter
Humans
Lipids
Luciferases
Nanoparticles
Particle Size
RNA Interference
RNA, Small Interfering
Transfection

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22838067