Abstract

OBJECTIVES
Ischemia-reperfusion (I/R) injury is the most common cause of renal dysfunction. Ischemic postconditioning (IPO) is a phenomenon by which intermittent interruptions of blood flow in the early phase of reperfusion protect an organ from I/R injury. In the present study, we investigated the effects of IPO on renal I/R injury using a canine autotransplantation model.
MATERIALS AND METHODS
Fifty adult male mongrel dogs were randomly divided into five groups of 10 animals each. The animals underwent a left nephrectomy followed by flushing and static preservation of the kidney in University of Wisconsin solution for 24 hours. IPO was performed by six cycles of 10 or 30 seconds or three cycles of 1-minute I/R before final reperfusion. All dogs underwent a right nephrectomy 24 hours later followed by autotransplantation of the preserved left kidney. Blood and urine were collected at various reperfusion times (24, 48, and 72 hours). We assayed blood urea nitrogen and creatinine levels, as well as urine N-acetyl-β-D-glucosaminidase levels. Kidney samples were harvested after I/R to measured renal superoxide dismutase (SOD), malondialdehyde (MDA), and myeloperoxidase (MPO) concentrations. Apoptosis was evaluated by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labeling assays of tissue samples.
RESULTS
Compared with the sham group, I/R resulted in renal dysfunction with decreased SOD and increased MDA and MPO levels, as well as increased apoptosis indices. However, IPO attenuated the above effects, particularly the six cycles of 10-second I/R.
CONCLUSIONS
IPO exerted protective effects on renal I/R injury.

Links

Publisher Full Text

Authors

Jiang B, Chen Q, Liu X, Kong D, Kuang Y, Weng X, Chen H

Institution

Department of Urology, Xianning Center Hospital, Xianning, China. doctorjbt@163.com

Source

Transplantation proceedings 44:6 pg 1776-81

MeSH

Acetylglucosaminidase
Adenosine
Allopurinol
Animals
Apoptosis
Biological Markers
Blood Urea Nitrogen
Cold Temperature
Creatinine
Dogs
Glutathione
Insulin
Ischemic Postconditioning
Kidney
Male
Malondialdehyde
Models, Animal
Nephrectomy
Organ Preservation
Organ Preservation Solutions
Peroxidase
Raffinose
Reperfusion Injury
Replantation
Superoxide Dismutase
Time Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22841271