Abstract

The effects of fat-soluble vitamins (such as vitamins A and E) and lipid microconstituents (such as carotenoids) on human health are now well established. However, high-performance liquid chromatography (HPLC) methods able to detect these molecules in simultaneous runs are often difficult to set up. We report here a 35-min reversed-phase HPLC method using a single C30 column kept at 35°C with a gradient system of methanol, methyl-tert-butyl ether and water at a flow-rate of 1 mL/min. This method resolves 11 carotenoids, retinol, α- and γ-tocopherol from complex matrixes such as food samples, human plasma and human adipose tissue within 35 min. The method is also able to separate coenzyme Q(10). The intra-day and inter-day coefficients of variation are suitable for routine clinical and scientific applications for the determination of lipid micronutrients from various sample types.

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Authors

Gleize B, Steib M, André M, Reboul E

Institution

INRA, UMR 1260 Nutriments Lipidiques et Prévention des Maladies Métaboliques, Marseille F-13385, France.

Source

Food chemistry 134:4 2012 Oct 15 pg 2560-4

MeSH

Adipose Tissue
Carotenoids
Chromatography, High Pressure Liquid
Chromatography, Reverse-Phase
Food Analysis
Humans
Tocopherols
Ubiquinone
Vitamin A

Pub Type(s)

Evaluation Studies
Journal Article

Language

eng

PubMed ID

22871234