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- Authors
- Wang Z
- Zhang Z
- Wu Y
- Chen L
- Luo Q
- Zhang J
- Chen J
- Luo Z
- MESH
- Animals
- Cells, Cultured
- Chorionic Gonadotropin
- Echinomycin
- Endothelin-2
- Female
- Gonadotropins, Equine
- Granulosa Cells
- Humans
- Hypoxia-Inducible Factor 1, alpha Subunit
- Oligonucleotides
- Ovary
- Rats
- Rats, Sprague-Dawley
- Superovulation
- Transcriptional Activation
Effects of echinomycin on endothelin-2 expression and ovulation in immature rats primed with gonadotropins.
Abstract
Echinomycin is a small-molecule inhibitor of hypoxia- inducible factor-1 DNA-binding activity, which plays a crucial role in ovarian ovulation in mammalians. The present study was designed to test the hypothesis that hypoxia-inducible factor (HIF)-1α-mediated endothelin (ET)-2 expressions contributed to ovarian ovulation in response to human chorionic gonadotropin (hCG) during gonadotropin-induced superuvulation. By real-time RT-PCR analysis, ET-2 mRNA level was found to significantly decrease in the ovaries after echinomycin treatment, while HIF-1α mRNA and protein expression was not obviously changed. Further analysis also showed that these changes of ET-2 mRNA were consistent with HIF-1 activity in the ovaires, which is similar with HIF-1α and ET-2 expression in the granulosa cells with gonadotropin and echinomycin treatments. The results of HIF-1α and ET-2 expression in the granulosa cells transfected with cis-element oligodeoxynucleotide (dsODN) under gonadotropin treatment further indicated HIF-1α directly mediated the transcriptional activation of ET-2 during gonadotropin- induced superuvulation. Taken together, these results demonstrated that HIF-1α-mediated ET-2 transcriptional activation is one of the important mechanisms regulating gonadotropin-induced mammalian ovulatory precess in vivo.
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Authors
Wang Z, Zhang Z, Wu Y, Chen L, Luo Q, Zhang J, Chen J, Luo Z, Huang X, Cheng Y
Institution
Provincial Key Laboratory for Developmental Biology and Neurosciences College of Life Sciences Fujian Normal University Fuzhou 350007, PR China. zcwang@fjnu.edu.cn
Source
Experimental & molecular medicine 44:10 2012 Oct 31 pg 615-21MeSH
AnimalsCells, Cultured
Chorionic Gonadotropin
Echinomycin
Endothelin-2
Female
Gonadotropins, Equine
Granulosa Cells
Humans
Hypoxia-Inducible Factor 1, alpha Subunit
Oligonucleotides
Ovary
Rats
Rats, Sprague-Dawley
Superovulation
Transcriptional Activation
Pub Type(s)
Journal ArticleResearch Support, Non-U.S. Gov't
Language
eng
PubMed ID
22874467