Abstract

2D-TLC and RP-HPLC methods were applied to qualitatively determinate free phenolic acids and those liberated by acid and alkaline hydrolysis in the flowers and leaves of G. robusta and G. squarrosa. The presence of eleven phenolic acids, namely: caffeic, chlorogenic, p-coumaric, p-hydroxybenzoic, ferulic, gallic, protocatechuic, vanillic salicylic, p-hydroxyphenylacetic and ellagic acids was determined. Quantitative estimate of phenolic acids, expressed as caffeic acid, has been analyzed by the method described in the Polish Pharmacopoeia VIII. The content of phenolic acids in G. robusta reached 7.33 mg/g and 6.23 mg/g for flowers and leaves, respectively. The flowers and leaves of G. squarrosa were characterized by similar level of phenolic acids, namely 6.81 mg/g and 6.59 mg/g, respectively.

Authors

Nowak S, Rychlińska I

Institution

Department of Pharmacognosy, Medical University of Łódź, 1 Muszyiskiego St., 90-151 Łódź, Poland. slawomira.nowak@umed.lodz.pl

Source

Acta poloniae pharmaceutica 69:4 pg 693-8

MeSH

Calibration
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Flowers
Grindelia
Hydrogen-Ion Concentration
Hydrolysis
Hydroxybenzoic Acids
Plant Leaves
Reference Standards

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22876612