MiR-195 regulates cell apoptosis of human hepatocellular carcinoma cells by targeting LATS2.
Recently, we have reported tissue- and stage-specific expression of miR-195 in human hepatocellular carcinoma cells and so far, not many reports discuss the function of this microRNA (miRNA). Expression profiling of miRNAs revealed a limited set of miRNAs with altered expression in drug resistant hepatocellular carcinoma cell line BEL-7402/5-FU compared to its parental BEL-7402 cell line. Real-time PCR confirmed down-regulation of miR-195 in BEL-7402/5-FU cells. Western blots were performed to determine protein levels of LATS2, P53 and CDK2. MTT analysed the cell proliferation activity. Flow cytometry were performed to determine apoptosis rate. Up-regulation of miR-195 increased expression of LATS2 and increased apoptosis of HCC cells, while Anti-miR-195 treatment inhibited expression of LATS2. miR-195 over-expression inhibited the luciferase activity of a LATS2 3' untranslated region-based reporter construct in BEL-7402/5-FU cells. These results indicate that miR-195 could increase cell apoptosis by targeting LATS2 in hepatocellular carcinoma cells.
Institute of Pharmacy and Pharmacology, The First Affilitated Hospital, University of South China Hengyang, P.R. China.
SourceDie Pharmazie 67:7 2012 Jul pg 645-51
MeSH3' Untranslated Regions
Cell Line, Tumor
Drug Resistance, Neoplasm
Nucleic Acid Hybridization
Real-Time Polymerase Chain Reaction
Tumor Suppressor Protein p53
Tumor Suppressor Proteins
Pub Type(s)Journal Article
Research Support, Non-U.S. Gov't