Abstract

Recently, we have reported tissue- and stage-specific expression of miR-195 in human hepatocellular carcinoma cells and so far, not many reports discuss the function of this microRNA (miRNA). Expression profiling of miRNAs revealed a limited set of miRNAs with altered expression in drug resistant hepatocellular carcinoma cell line BEL-7402/5-FU compared to its parental BEL-7402 cell line. Real-time PCR confirmed down-regulation of miR-195 in BEL-7402/5-FU cells. Western blots were performed to determine protein levels of LATS2, P53 and CDK2. MTT analysed the cell proliferation activity. Flow cytometry were performed to determine apoptosis rate. Up-regulation of miR-195 increased expression of LATS2 and increased apoptosis of HCC cells, while Anti-miR-195 treatment inhibited expression of LATS2. miR-195 over-expression inhibited the luciferase activity of a LATS2 3' untranslated region-based reporter construct in BEL-7402/5-FU cells. These results indicate that miR-195 could increase cell apoptosis by targeting LATS2 in hepatocellular carcinoma cells.

Authors

Yang X, Yu J, Yin J, Xiang Q, Tang H, Lei X

Institution

Institute of Pharmacy and Pharmacology, The First Affilitated Hospital, University of South China Hengyang, P.R. China.

Source

Die Pharmazie 67:7 2012 Jul pg 645-51

MeSH

3' Untranslated Regions
Antimetabolites
Apoptosis
Blotting, Western
Carcinoma, Hepatocellular
Cell Line, Tumor
Cell Proliferation
Down-Regulation
Drug Resistance, Neoplasm
Fluorouracil
Humans
MicroRNAs
Microarray Analysis
Nucleic Acid Hybridization
Plasmids
Protein-Serine-Threonine Kinases
Real-Time Polymerase Chain Reaction
Transfection
Tumor Suppressor Protein p53
Tumor Suppressor Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22888524