Gene sequencing for routine verification of pyrazinamide resistance in Mycobacterium tuberculosis: a role for pncA but not rpsA.
Pyrazinamide (PZA) is an important component of first-line therapy for the treatment of tuberculosis. Here, we evaluate targeted gene sequencing as a supplement to phenotypic PZA susceptibility testing of Mycobacterium tuberculosis. Routine sequencing of pncA, but not rpsA, is effective for verification of PZA susceptibility results.
Public Health Ontario, Public Health Laboratories, Toronto, Ontario, Canada.
SourceJournal of clinical microbiology 50:11 2012 Nov pg 3726-8
Drug Resistance, Bacterial
Microbial Sensitivity Tests
Sequence Analysis, DNA
Pub Type(s)Evaluation Studies