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Gene sequencing for routine verification of pyrazinamide resistance in Mycobacterium tuberculosis: a role for pncA but not rpsA.

Abstract

Pyrazinamide (PZA) is an important component of first-line therapy for the treatment of tuberculosis. Here, we evaluate targeted gene sequencing as a supplement to phenotypic PZA susceptibility testing of Mycobacterium tuberculosis. Routine sequencing of pncA, but not rpsA, is effective for verification of PZA susceptibility results.

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    • Authors

    Alexander DC, Ma JH, Guthrie JL, Blair J, Chedore P, Jamieson FB

    Institution

    Public Health Ontario, Public Health Laboratories, Toronto, Ontario, Canada.

    Source

    Journal of clinical microbiology 50:11 2012 Nov pg 3726-8

    MeSH

    Amidohydrolases
    Antitubercular Agents
    DNA, Bacterial
    Drug Resistance, Bacterial
    Humans
    Microbial Sensitivity Tests
    Mycobacterium tuberculosis
    Pyrazinamide
    Sequence Analysis, DNA
    Tuberculosis

    Pub Type(s)

    Evaluation Studies
    Journal Article

    Language

    eng

    PubMed ID

    22895038