Label-free imaging of cerebral β-amyloidosis with extended-focus optical coherence microscopy.
We demonstrate label-free imaging of cerebral β-amyloidosis ex vivo and in a living mouse model of Alzheimer's disease using extended-focus Fourier domain optical coherence microscopy (xfOCM). xfOCM provides 3D, high-resolution images of individual β-amyloid plaques in the brain parenchyma and vasculature and requires no staining of the alzheimeric sample under investigation. xfOCM also opens the possibility to perform minimally invasive studies of β-amyloid pathology in vivo, without the use of labeling methods, which potentially confound experimental findings.
Laboratory of Molecular and Cellular Biology of Alzheimer's Disease, Brain Mind Institute and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH1015 Lausanne, Switzerland.
SourceThe Journal of neuroscience : the official journal of the Society for Neuroscience 32:42 2012 Oct 17 pg 14548-56
Cerebral Amyloid Angiopathy
Disease Models, Animal
Staining and Labeling
Tomography, Optical Coherence
Pub Type(s)Journal Article
Research Support, Non-U.S. Gov't